6JRF

Crystal structure of ZmMoc1-Holliday junction Complex in the presence of Calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	51.850, 78.164, 64.033
Unit cell angles	90.00, 97.39, 90.00

Refinement procedure

Resolution	63.501 - 2.047
R-factor	0.2112
Rwork	0.208
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6is9
Data reduction software	DENZO
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.501	63.500	2.160
High resolution limit [Å]	2.047	6.470	2.050
Rmerge	0.069	0.057	0.690
Rmeas	0.075	0.062	0.746
Rpim	0.028	0.024	0.281
Number of reflections	31763	1029	4663
<I/σ(I)>	12.6
Completeness [%]	99.2	97.9	99.8
Redundancy	6.8	6.4	7.1
CC(1/2)	0.998	0.997	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	50mM CaCl2, 10mM MES pH 5.5, 40% PEG 200