6JR7
Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 116.591, 102.602, 114.130 |
Unit cell angles | 87.05, 113.07, 118.53 |
Refinement procedure
Resolution | 35.150 - 1.750 |
R-factor | 0.1633 |
Rwork | 0.162 |
R-free | 0.18550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jr6 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.736 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.200 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.050 | 0.353 |
Number of reflections | 430825 | 19944 |
<I/σ(I)> | 20.5 | 2.5 |
Completeness [%] | 94.8 | 88 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM sodium acetate buffer, 8% (w/v) polyethylene glycol 20000, and 8% 2-methyl-2,4-pentanediol |