6JQV

Crystal structure of Arabidopsis thaliana NRP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9794
Spacegroup name	H 3 2
Unit cell lengths	123.450, 123.450, 228.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.040 - 3.420
R-factor	0.2746
Rwork	0.272
R-free	0.32020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5day
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.040	3.544
High resolution limit [Å]	3.420	3.422
Rmerge	0.128
Rmeas	0.134
Rpim	0.040	0.370
Number of reflections	9304	914
<I/σ(I)>	17.37	2.31
Completeness [%]	99.7	100
Redundancy	11.4	12
CC(1/2)	0.999	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M potassium fluoride, 2.2M Ammonium sulfate