6JQF

Crystallization analysis of a beta-N-acetylhexosaminidase (Am2136) from Akkermansia muciniphila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	108.819, 114.548, 128.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.720 - 1.900
R-factor	0.1604
Rwork	0.159
R-free	0.18920
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MoRDa
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.128	0.098	1.099
Rmeas	0.133	0.103	1.148
Rpim	0.038	0.029	0.331
Number of reflections	62210	10652	10338
<I/σ(I)>	8.9
Completeness [%]	99.8	98.8	100
Redundancy	12.2	12.5	11.9
CC(1/2)		0.992	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5; 25% PEG3350