6JQ2
Crystal Structure of H2-Kb in complex with a DPAGT1 self-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-01-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.240, 69.420, 70.440 |
| Unit cell angles | 90.00, 103.79, 90.00 |
Refinement procedure
| Resolution | 57.760 - 2.400 |
| R-factor | 0.2261 |
| Rwork | 0.221 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.059 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.760 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.023 | 0.116 |
| Number of reflections | 19013 | 1886 |
| <I/σ(I)> | 14.38 | |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 4% v/v Tacsimate (pH 573 7.0), 12% w/v Polyethylene glycol 3,350 |
