6JQ1
Crystal Structure of DdrO from Deinococcus geothermalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2016-10-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.950, 127.900, 156.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.238 |
| Rwork | 0.237 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pu7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.049 | 0.692 |
| Number of reflections | 16314 | 1177 |
| <I/σ(I)> | 16.88 | |
| Completeness [%] | 98.7 | 96.9 |
| Redundancy | 4.67 | 4.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | LiCl, HEPES |
