6JPM

Crystal Structure of Odorant Binding Protein 4 in the Natural Predator Chrysopa pallens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	120
Detector technology	PIXEL
Collection date	2018-10-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 41
Unit cell lengths	135.745, 135.745, 29.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.993 - 2.098
R-factor	0.1835
Rwork	0.182
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.007
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc3_3206: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.993	2.160
High resolution limit [Å]	2.098	2.098
Number of reflections	16276
<I/σ(I)>	8.1
Completeness [%]	99.3	94
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	30%(V/V) 2-propanol, 100mM Tris pH 7.5, 33%(W/V) PEG3350