6JPH
Crystal structure of the catalytic domain of a multi-domain alginate lyase Dp0100 from thermophilic bacterium Defluviitalea phaphyphila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-23 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 261.923, 261.923, 58.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 131.305 - 2.759 |
| Rwork | 0.199 |
| R-free | 0.22550 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.284 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.305 | 2.810 |
| High resolution limit [Å] | 2.759 | 2.760 |
| Rmeas | 0.287 | 2.497 |
| Rpim | 0.086 | 0.740 |
| Number of reflections | 58914 | 2878 |
| <I/σ(I)> | 6.2 | 0.9 |
| Completeness [%] | 100.0 | 99.2 |
| Redundancy | 11 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 298 | 15% PEG 6000, 0.1 M magnesium acetate |
