6JOZ
Crystal structure of BRLF peptide from EBV in complex with HLA-A1101.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.395, 72.931, 125.699 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.080 - 1.350 |
| R-factor | 0.16183 |
| Rwork | 0.160 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x7q |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.080 | 1.385 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.080 | 0.561 |
| Number of reflections | 101182 | |
| <I/σ(I)> | 2.31 | |
| Completeness [%] | 97.0 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.918 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M LITHIUM SULFATE MONOHYDRATE, 0.1M TRIS-HCL, 30% PEG 4000 |
