6JOM

Crystal structure of lipoate protein ligase from Mycoplasma hyopneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97894
Spacegroup name	P 41 21 2
Unit cell lengths	100.322, 100.322, 155.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.150 - 2.450
R-factor	0.204
Rwork	0.201
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.753
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.450	5.280	2.450
Rmerge	0.060	0.034	0.758
Rmeas	0.062	0.036	0.785
Rpim	0.016	0.009	0.193
Number of reflections	31325	3375	3049
<I/σ(I)>	13.4
Completeness [%]	99.6	99.3	99.1
Redundancy	14.9	15.1	14.9
CC(1/2)		0.999	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	289	0.2 M (NH4)2SO4, 0.1 M sodium acetate pH 4.0, 20% PEG 2000 MME
2