6JOH
The crystal of nucleoside diphosphate kinase from Aspergillus flavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.836, 169.470, 146.937 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 33.690 - 2.400 |
| R-factor | 0.22404 |
| Rwork | 0.222 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nsk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.628 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.670 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 179636 | 17977 |
| <I/σ(I)> | 49.1 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 2.4M Sodium Citrate, 100mM HEPES, pH 8.5 |
