6JOB

Ferritin variant with "GMG" motif

Refinement Statistics

Experimental method: SOLUTION SCATTERING, X-RAY DIFFRACTION (2.93 Å)

Cell axes	219.170	219.170	147.950
Cell angles	90.00	90.00	90.00
Spacegroup	P 42 21 2
Resolution limits	107.01 - 2.93

Crystallization Conditions

crystal ID	method	pH	pH range	temperature	unit
1	VAPOR DIFFUSION, HANGING DROP			293.15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	77.15
Detector technology	CCD
Collection date	2018-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97894
Spacegroup name	P 42 21 2
Unit cell lengths	219.170, 219.170, 147.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.014 - 2.930
R-factor	0.2882
Rwork	0.284
R-free	0.36770
RMSD bond length	0.012
RMSD bond angle	1.569
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.014	3.050
High resolution limit [Å]	2.930	2.970
Number of reflections	76098
<I/σ(I)>	7
Completeness [%]	98.0
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	sodium citrate tribase dihydrate, 1,6-Hexanediol