6JO3
Crystal structure of (S)-3-O-geranylgeranylglyceryl phosphate synthase from Thermoplasma acidophilum in complex with substrate sn-glycerol-1-phosphate
Replaces: 5B69Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2006-06-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.595, 105.595, 142.498 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.538 - 2.350 |
R-factor | 0.1997 |
Rwork | 0.199 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mm1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.522 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 20203 | 1945 |
<I/σ(I)> | 8.3 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.6 | 10.9 |
CC(1/2) | 0.988 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 0.1 M sodium acetate pH 4.8 10% (w/v) polyethylene glycol 4000 10 mM rac-G1P 100 mM cesium chloride |