6JO2
Ferredoxin I from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.172, 53.222, 32.293 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 38.610 - 1.550 |
| R-factor | 0.1349 |
| Rwork | 0.133 |
| R-free | 0.17590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aui |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.408 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.610 | 38.610 | 1.640 |
| High resolution limit [Å] | 1.550 | 4.610 | 1.550 |
| Number of reflections | 13408 | 1983 | |
| <I/σ(I)> | 12.04 | 2.9 | |
| Completeness [%] | 96.5 | 90.1 | |
| Redundancy | 3.3 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Sodium Citrate (pH 6.5) 2.5 M ammonium Sulfate 0.2%(w/v) benzamidine hydrochloride 3%(w/v) 1,6-hexanediol |
