6JMU
Crystal structure of GIT1/Paxillin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.200, 90.954, 52.309 |
| Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
| Resolution | 45.477 - 2.000 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jx0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.114 | 0.043 | 0.764 |
| Rmeas | 0.124 | 0.047 | 0.827 |
| Rpim | 0.047 | 0.017 | 0.312 |
| Total number of observations | 126793 | ||
| Number of reflections | 18573 | 957 | 904 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 98.6 | 97.8 | 98.8 |
| Redundancy | 6.8 | 6.8 | 6.8 |
| CC(1/2) | 1.000 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | (NH4)2SO4, PEG4000 |
