6JMB
Crystal structure of Ostrinia furnacalis Group IV chitinase in complex with allosamidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.625, 71.707, 72.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.431 - 1.389 |
| R-factor | 0.1497 |
| Rwork | 0.149 |
| R-free | 0.15950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wup |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.854 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.389 | 1.390 |
| Number of reflections | 124774 | 5609 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 20%(v/v) 1,4-butanediol, 100mM sodium acetate, 100mM acetic acid, pH 4.5 |
