6JM8
Crystal structure of Ostrinia furnacalis Group IV chitinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-14 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.573, 73.781, 89.414 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.679 - 1.911 |
R-factor | 0.1784 |
Rwork | 0.177 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wup |
RMSD bond length | 0.004 |
RMSD bond angle | 0.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.911 | 1.911 |
Number of reflections | 43869 | 3321 |
<I/σ(I)> | 4.8 | 3.87 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 7.8 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 277 | 1600 mM sodium phosphate monobasic, 400 mM potassium phosphate dibasic, 100 mM sodium phosphate dibasic, 100 mM citric acid, pH 4.7 |