6JM7
Crystal structure of Ostrinia furnacalis Group IV chitinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.734, 71.593, 72.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.797 - 1.572 |
R-factor | 0.1592 |
Rwork | 0.159 |
R-free | 0.17070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wup |
RMSD bond length | 0.007 |
RMSD bond angle | 0.891 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 56099 | 2729 |
<I/σ(I)> | 10.3 | 5.45 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 9.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | 1600 mM sodium phosphate monobasic, 400 mM potassium phosphate dibasic, 100 mM sodium phosphate dibasic, 100 mM citric acid, pH 4.2 |