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6JLO

XFEL structure of cyanobacterial photosystem II (2F state, dataset2)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeFREE ELECTRON LASER
Source detailsSACLA BEAMLINE BL3
Synchrotron siteSACLA
BeamlineBL3
Temperature [K]100
Detector technologyCCD
Collection date2017-04-09
DetectorRAYONIX MX300-HS
Wavelength(s)1.2398
Spacegroup nameP 21 21 21
Unit cell lengths122.040, 228.840, 286.980
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.988 - 2.400
R-factor0.1541
Rwork0.151
R-free0.20730
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6jlj
RMSD bond length0.010
RMSD bond angle1.403
Data reduction softwareCrystFEL
Data scaling softwareCrystFEL
Phasing softwarePHASER
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.490
High resolution limit [Å]2.4002.400
Number of reflections32840232568
<I/σ(I)>9.6
Completeness [%]100.0
Redundancy955
CC(1/2)0.9970.321
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1BATCH MODE293PEG, magnesium sulfate

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