6JLI

Crystal structure of CTLD7 domain of human PLA2R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	70.652, 70.652, 64.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.326 - 1.778
R-factor	0.1641
Rwork	0.162
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xts
RMSD bond length	0.015
RMSD bond angle	1.304
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.330	1.842
High resolution limit [Å]	1.778	1.778
Rmerge	0.090	0.280
Number of reflections	18297	1775
<I/σ(I)>	30.95
Completeness [%]	99.8
Redundancy	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	20% PEG3350, 0.2M Ammonium sulfate pH6.0