6JLF
Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae D179A mutation - HR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.970, 159.468, 161.901 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 93.195 - 2.548 |
R-factor | 0.2307 |
Rwork | 0.230 |
R-free | 0.27590 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.213 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.195 | 2.640 |
High resolution limit [Å] | 2.548 | 2.548 |
Number of reflections | 95113 | |
<I/σ(I)> | 18.74 | |
Completeness [%] | 98.2 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05M ZnAce, 6.6% PEG 8000, 0.1M Na-Cacodylat (pH 6.0), 6% EG |