6JLC
Structure determination of CAMP factor of Mobiluncus curtisii and insight into structural dynamics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2014-07-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 75.670, 75.670, 192.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.799 - 1.847 |
| R-factor | 0.1972 |
| Rwork | 0.196 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h6i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.671 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.799 | 1.900 |
| High resolution limit [Å] | 1.847 | 1.850 |
| Rmeas | 0.007 | 0.990 |
| Number of reflections | 28725 | 2010 |
| <I/σ(I)> | 32.75 | |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 18.09 | 15 |
| CC(1/2) | 1.000 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 18% PEG 8000, 0.1M ammonium acetate, 0.1M sodium acetate, pH 5.0 |
