6JLB

Crystal structure of lamin A/C fragment and assembly mechanisms of intermediate filaments

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 2
Unit cell lengths	231.267, 84.997, 92.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.191 - 3.205
R-factor	0.2112
Rwork	0.207
R-free	0.26050
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.453
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.108	0.368
Rmeas	0.117
Rpim	0.043	0.207
Number of reflections	40335
<I/σ(I)>	11.5	3.1
Completeness [%]	93.5	80.8
Redundancy	5.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	287	0.1 M Tris-HCl (pH 8.5), 0.9 M Lithium chloride, 7% (v/v) PEG 8000