6JLA

Crystal structure of a mouse ependymin related protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00065
Spacegroup name	P 1 21 1
Unit cell lengths	56.966, 59.671, 137.336
Unit cell angles	90.00, 101.29, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.18766
Rwork	0.185
R-free	0.24007
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	35643
<I/σ(I)>	44.4
Completeness [%]	99.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Sodium citrate tribasic dihydrate pH 5.6, 0.5 M Ammonium sulfate, 1.0 M Lithium sulfate monohydrate