6JLA
Crystal structure of a mouse ependymin related protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00065 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.966, 59.671, 137.336 |
Unit cell angles | 90.00, 101.29, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.18766 |
Rwork | 0.185 |
R-free | 0.24007 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 35643 | |
<I/σ(I)> | 44.4 | |
Completeness [%] | 99.9 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 0.5 M Ammonium sulfate, 1.0 M Lithium sulfate monohydrate |