6JL9

Crystal structure of a frog ependymin related protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.540
Spacegroup name	P 65 2 2
Unit cell lengths	61.208, 61.208, 236.196
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.010 - 2.000
R-factor	0.19819
Rwork	0.194
R-free	0.27753
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.740
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.010	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	18642
<I/σ(I)>	70.5
Completeness [%]	99.7
Redundancy	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M Calcium acetate hydrate, 0.1M Sodium cacodylate trihydrate pH 6.5, 18%(w/v) Polyethylene glycol 8,000