6JL7
crystal structure of TBC1D23 N terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 65 |
Unit cell lengths | 151.736, 151.736, 38.604 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.720 - 2.500 |
R-factor | 0.2191 |
Rwork | 0.217 |
R-free | 0.25190 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.742 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.158 | 0.111 | |
Rmeas | 0.159 | 0.115 | |
Rpim | 0.051 | 0.031 | 0.537 |
Number of reflections | 17980 | 972 | 903 |
<I/σ(I)> | 3.5 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 14.1 | 13.6 | 14 |
CC(1/2) | 0.994 | 0.453 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M MES pH6.5, 0.5 M Ammonium nitrate, 25% polyethylene glycol 400 |