6JL1
Crystal structure of VvPlpA G389D from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.397, 54.397, 233.181 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.100 - 2.290 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jkz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.103 | 2.327 |
High resolution limit [Å] | 2.288 | 2.288 |
Rmeas | 0.097 | 0.734 |
Rpim | 0.023 | 0.180 |
Number of reflections | 19033 | 917 |
<I/σ(I)> | 17.3 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.6 | 16.3 |
CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5 mg/ml in 10 mM HEPES, 150 mM NaCl, pH 7.5 was mixed with equal volume of reservoir solution (200 mM potassium sodium tartrate tetrahydrate, 180 mM NDSB-201, 8% polyethylene glycol 1500, and 20% glycerol) |