6JKZ

Crystal structure of VvPlpA from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	54.158, 54.158, 233.143
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.981 - 1.397
R-factor	0.1666
Rwork	0.166
R-free	0.18740
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.902	1.421
High resolution limit [Å]	1.397	1.397
Rmeas	0.069	0.926
Rpim	0.016	0.255
Number of reflections	79933	3826
<I/σ(I)>	22
Completeness [%]	99.7	96.6
Redundancy	18.8	12.8
CC(1/2)	0.998	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 ul of protein solution (8 mg/ml in the buffer 10 mM HEPES, 150 mM NaCl, pH 7.5) and 1.5 ul of reservoir solution containing 500 mM NaCl, 10 mM MgCl2, 180 mM NDSB-201, 30% glycerine.