6JKY

Crystal structure of MvcA-UBE2N-Ub complex from Legionella pneumophila

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97894
Spacegroup name	C 2 2 21
Unit cell lengths	104.325, 107.605, 263.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.135 - 2.454
R-factor	0.2111
Rwork	0.209
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6K11 and 1JAT
RMSD bond length	0.004
RMSD bond angle	0.704
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.140	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.066	0.547
Rmeas	0.071	0.591
Number of reflections	52753	7924
<I/σ(I)>	14.9
Completeness [%]	96.5
Redundancy	6.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Sodium thiocyanate, 20% w/v Polyethylene glycol 3,350