6JKU
Crystal structure of N-acetylglucosamine-6-phosphate deacetylase from Pasteurella Multocida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9750 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.162, 139.549, 166.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.129 - 1.950 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p50 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.112 | |
| Rmeas | 0.124 | |
| Rpim | 0.052 | 0.526 |
| Number of reflections | 134675 | 6848 |
| <I/σ(I)> | 10 | 1.9 |
| Completeness [%] | 89.8 | 92.7 |
| Redundancy | 4.9 | 4.8 |
| CC(1/2) | 0.997 | 0.636 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES sodium salt pH 7.5, 0.8 M Sodium phosphate |
