6JKQ
Crystal structure of aspartate transcarbamoylase from Trypanosoma cruzi (Ligand-free form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-22 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.419, 79.282, 92.016 |
| Unit cell angles | 69.56, 82.90, 63.25 |
Refinement procedure
| Resolution | 29.510 - 2.810 |
| R-factor | 0.18214 |
| Rwork | 0.178 |
| R-free | 0.26547 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ml4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | 0.389 |
| Number of reflections | 44850 | 4436 |
| <I/σ(I)> | 15.8 | 4.4 |
| Completeness [%] | 97.7 | 98.4 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 10% PEG 3350, 0.1 M acetate buffer pH 4.6, 0.2 M ammonium acetate, 0.01 M cobalt chloride and 3% glycerol |
