6JKL
Structure of a triple-helix region of human collagen type II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-27 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 60.541, 17.424, 51.637 |
Unit cell angles | 90.00, 114.39, 90.00 |
Refinement procedure
Resolution | 29.737 - 2.148 |
R-factor | 0.1983 |
Rwork | 0.196 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.189 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.737 | 2.190 |
High resolution limit [Å] | 2.148 | 2.150 |
Number of reflections | 2848 | |
<I/σ(I)> | 24.79 | |
Completeness [%] | 99.3 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M NaCl, 0.1 M Imidazole:HCl pH 8.0, 30%(w/v) PEG 8000 |