6JKC
Crystal structure of tetrameric PepTSo2 in P4212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 119.410, 119.410, 104.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 3.500 |
| R-factor | 0.269 |
| Rwork | 0.267 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lep |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.554 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 3.710 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.284 | 1.359 |
| Rmeas | 0.328 | 1.586 |
| Number of reflections | 8312 | 1295 |
| <I/σ(I)> | 5.49 | |
| Completeness [%] | 83.0 | 83.4 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.961 | 0.293 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4 | 293 | 40% PEG 200, 100 mM NaCl, 100 mM Na acetate, pH 4.0 |
