6JK3
Crystal structure of a mini fungal lectin, PhoSL in complex with core-fucosylated chitobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-18 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.99984 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.295, 61.295, 67.916 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.430 - 1.120 |
R-factor | 0.12211 |
Rwork | 0.121 |
R-free | 0.14506 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jk2 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.620 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.160 |
High resolution limit [Å] | 1.120 | 1.120 |
Rmerge | 0.062 | 0.649 |
Number of reflections | 50196 | 4797 |
<I/σ(I)> | 42.85 | 2.22 |
Completeness [%] | 99.6 | 96.9 |
Redundancy | 12.4 | 4.3 |
CC(1/2) | 0.943 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 1.6 M Sodium citrate pH 6.5 |