6JK2

Crystal structure of a mini fungal lectin, PhoSL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-07
Detector	RAYONIX MX300-HS
Wavelength(s)	1.7
Spacegroup name	P 63
Unit cell lengths	42.475, 42.475, 37.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.250 - 1.060
R-factor	0.13695
Rwork	0.136
R-free	0.15798
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.623
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.100
High resolution limit [Å]	1.060	1.060
Rmerge	0.036	0.213
Number of reflections	17498	1685
<I/σ(I)>	77.87	14.41
Completeness [%]	99.3	96.2
Redundancy	21.2	19.1
CC(1/2)	0.997	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.2M Ammonium sulfate, 30% w/v Polyethylene glycol 8000