6JK1
Crystal Structure of YAP1 and Dendrin complex 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97855 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.376, 77.376, 116.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.191 - 2.000 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jk0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.698 |
| Number of reflections | 24702 | 1210 |
| <I/σ(I)> | 32.8 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 28-35% w/v pentaerythritol propoxylat 426 (5/4 PO/OH), 100mM HEPES (pH 7.5) |
