6JK1
Crystal Structure of YAP1 and Dendrin complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.376, 77.376, 116.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.191 - 2.000 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.21110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jk0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.103 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.079 | 0.698 |
Number of reflections | 24702 | 1210 |
<I/σ(I)> | 32.8 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.8 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 28-35% w/v pentaerythritol propoxylat 426 (5/4 PO/OH), 100mM HEPES (pH 7.5) |