6JJX

Crystal Structure of KIBRA and Angiomotin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 2
Unit cell lengths	65.627, 72.283, 79.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.687 - 2.000
R-factor	0.2096
Rwork	0.208
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j68
RMSD bond length	0.008
RMSD bond angle	1.142
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.103	0.752
Number of reflections	26165	1282
<I/σ(I)>	22.4	2.2
Completeness [%]	99.8	99.9
Redundancy	9.9	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	25% w/v pentaerythritol propoxylat 629 (17/8 PO/OH), 50mM MgCl2, 100mM Tris (pH 8.5)