6JJG
Crystal structure of a two-quartet DNA mixed-parallel/antiparallel G-quadruplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97907 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 48.654, 46.807, 36.843 |
Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
Resolution | 23.075 - 1.969 |
R-factor | 0.2556 |
Rwork | 0.254 |
R-free | 0.27430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jje |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.672 | 2.080 |
High resolution limit [Å] | 1.969 | 1.969 |
Rpim | 0.025 | 0.196 |
Number of reflections | 5421 | |
<I/σ(I)> | 15.52 | |
Completeness [%] | 97.7 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | Potassium cacodylate, Potassium chloride, Sodium cacodylate, Spermine, Potassium bromide, MPD |