6JJ7
Crystal structure of OsHXK6-Glc complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2018-06-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 131.569, 131.569, 188.782 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.470 - 2.900 |
R-factor | 0.2019 |
Rwork | 0.200 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qs7 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.460 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.470 | 3.004 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.031 | 0.145 |
Number of reflections | 42411 | 4180 |
<I/σ(I)> | 15.15 | 4.54 |
Completeness [%] | 100.0 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M lithium sulfate, 0.1 M ADA 6.5, 30% v/v PEG 400, and the additive 29 (C5) from kit Additive ScreenTM HR2-428 (Hampton Research),2mM glucose |