6JJ4
Crystal structure of OsHXK6-apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-10-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.890, 65.890, 170.621 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.280 - 2.600 |
| R-factor | 0.2569 |
| Rwork | 0.250 |
| R-free | 0.31910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qs8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.099 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.280 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.027 | 0.059 |
| Number of reflections | 13424 | 1324 |
| <I/σ(I)> | 14.81 | 8.16 |
| Completeness [%] | 97.0 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M ammonium tartrate dibasic, and 20% (w/v) PEG 3,350 |
