6JIX
The cyrstal structure of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense, in complex with PLP and glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.390, 164.544, 109.240 |
Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
Resolution | 44.289 - 2.647 |
R-factor | 0.1687 |
Rwork | 0.167 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lh9 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 7.190 | 2.700 |
High resolution limit [Å] | 2.640 | 5.710 | 2.650 |
Rmerge | 0.112 | 0.067 | 0.490 |
Rmeas | 0.133 | 0.079 | 0.578 |
Rpim | 0.071 | 0.042 | 0.304 |
Number of reflections | 66599 | 3354 | 3302 |
<I/σ(I)> | 4 | ||
Completeness [%] | 98.9 | 99.4 | 98.5 |
Redundancy | 3.4 | 3.5 | 3.5 |
CC(1/2) | 0.994 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.1M MES, pH 5.0, 0.2M ammonium chloride, 10% W/V PEG 6000, 0.5mM PLP, 100mM glutamate |