6JIM
Viral helicase protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-04-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.888, 53.905, 100.718 |
| Unit cell angles | 96.93, 94.07, 97.04 |
Refinement procedure
| Resolution | 35.320 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.895 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.324 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 73699 | 7327 |
| <I/σ(I)> | 1 | |
| Completeness [%] | 97.7 | |
| Redundancy | 4 | |
| CC(1/2) | 0.995 | 0.344 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 100 mM HEPES pH 7.5, 20 % PEG 8000 |
