6JIK
Aspergillus fumigatus Rho1 GsGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-01-08 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.250, 65.700, 75.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.110 - 2.350 |
R-factor | 0.23448 |
Rwork | 0.232 |
R-free | 0.27505 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zvp |
RMSD bond length | 0.002 |
RMSD bond angle | 1.214 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.110 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.192 | 0.370 |
Rpim | 0.115 | 0.246 |
Number of reflections | 8136 | 767 |
<I/σ(I)> | 5.8 | 3.2 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 6.5 | 5.3 |
CC(1/2) | 0.982 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | crystals were obtained in Morpheus commercial crystallization screen, Imidazole and MES monohydrate (acid) at pH 6.5, 40% v/v PEG 500 MME and 20 % w/v PEG 20000, and 0.2M 1,6-Hexanediol, 0.2M 1-Butanol 0.2M 1,2-Propanediol, 0.2M 2-Propanol, 0.2M 1,4- Butanediol and 0.2M 1,3-Propanediol. |