6JHO
The complex crystal structure of Cagbeta with CagZ revealed a novel regulatory mechanism for T4SS coupling ATPase in Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97775 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.298, 96.298, 212.658 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.149 - 2.100 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s2x |
RMSD bond length | 0.008 |
RMSD bond angle | 0.826 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.083 | 0.034 | 1.562 |
Rmeas | 0.087 | 0.036 | 1.639 |
Rpim | 0.024 | 0.010 | 0.488 |
Number of reflections | 68411 | 3768 | 3339 |
<I/σ(I)> | 4 | ||
Completeness [%] | 99.9 | 100 | 99 |
Redundancy | 12.3 | 12.2 | 10.3 |
CC(1/2) | 0.999 | 0.543 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | CAPSO, PEG8k, sodium chloride |