6JHJ
Structure of Marine bacterial laminarinase mutant-E135A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-11-17 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.808, 94.606, 72.442 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.607 - 1.690 |
R-factor | 0.15191 |
Rwork | 0.150 |
R-free | 0.18483 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4crq |
RMSD bond length | 0.019 |
RMSD bond angle | 1.725 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 1.790 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.044 | 0.593 |
Number of reflections | 22574 | 945 |
<I/σ(I)> | 24.2 | |
Completeness [%] | 96.5 | 73.9 |
Redundancy | 6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% (v/v) ethonal, 28% PEG20000 100 mM MES pH7.0 |