6JGV
Crystal structure of the transcriptional regulator CadR from P. putida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 |
Unit cell lengths | 47.304, 47.304, 100.385 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.24983 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jgf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.243 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.307 | |
Number of reflections | 12450 | 1228 |
<I/σ(I)> | 21.1 | 3.7 |
Completeness [%] | 97.5 | 95.3 |
Redundancy | 5.9 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | PEG 3350, magnesum chloride, Tris-HCl |