6JGI
Crystal structure of the S65T/F99S/M153T/V163A variant of GFP at 0.85 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 56 |
Detector technology | CCD |
Collection date | 2016-05-18 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.75 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.857, 62.403, 69.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.334 - 0.850 |
R-factor | 0.0932 |
Rwork | 0.092 |
R-free | 0.11180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wur |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 0.810 |
High resolution limit [Å] | 0.800 | 2.170 | 0.800 |
Rmerge | 0.090 | 0.062 | 1.661 |
Rmeas | 0.099 | 0.067 | 1.847 |
Rpim | 0.039 | 0.026 | 0.791 |
Number of reflections | 226086 | 11828 | 11137 |
<I/σ(I)> | 7.8 | ||
Completeness [%] | 97.8 | 97.4 | 97.5 |
Redundancy | 5.7 | 6.5 | 5.2 |
CC(1/2) | 0.994 | 0.295 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 308 | PEG 4000, MgCl2, Tris-HCl buffer |