6JG8

Crystal structure of AimR in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	98.007, 98.007, 159.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.273 - 2.097
R-factor	0.2109
Rwork	0.210
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xyb
RMSD bond length	0.009
RMSD bond angle	1.066
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc3_3206: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.130
High resolution limit [Å]	2.097	2.097
Number of reflections	97895
<I/σ(I)>	1.7
Completeness [%]	99.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	sodium cacodylate, PEG 4000