6JFP
Crystal structure of the beta-glucosidase Bgl15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.296, 93.353, 94.057 |
| Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
| Resolution | 49.729 - 2.700 |
| R-factor | 0.2205 |
| Rwork | 0.219 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gnx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.135 | 0.230 |
| Number of reflections | 24023 | 2101 |
| <I/σ(I)> | 12.6 | 3.8 |
| Completeness [%] | 96.3 | 88.9 |
| Redundancy | 3 | 2.3 |
| CC(1/2) | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2M NH4Cl, 0.1M Tris-HCl (pH 8.0), 20% PEG 3350 |
