6JFM

Mitofusin2 (MFN2)_T111D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97914
Spacegroup name	P 1 21 1
Unit cell lengths	44.437, 126.563, 79.972
Unit cell angles	90.00, 102.46, 90.00

Refinement procedure

Resolution	31.641 - 2.090
R-factor	0.1784
Rwork	0.176
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jfk
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.600	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.070	0.573
Rmeas	0.090	0.743
Number of reflections	50379	3741
<I/σ(I)>	10.6
Completeness [%]	98.8
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.16 M Calcium acetate 0.08 M Sodium cacodylate PH 6.5 14.4% (W/V) PEG 8000 20% (V/V) Glycerol