6JFM
Mitofusin2 (MFN2)_T111D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97914 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.437, 126.563, 79.972 |
Unit cell angles | 90.00, 102.46, 90.00 |
Refinement procedure
Resolution | 31.641 - 2.090 |
R-factor | 0.1784 |
Rwork | 0.176 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jfk |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.600 | 2.140 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.070 | 0.573 |
Rmeas | 0.090 | 0.743 |
Number of reflections | 50379 | 3741 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 98.8 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.16 M Calcium acetate 0.08 M Sodium cacodylate PH 6.5 14.4% (W/V) PEG 8000 20% (V/V) Glycerol |