6JFM
Mitofusin2 (MFN2)_T111D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.437, 126.563, 79.972 |
| Unit cell angles | 90.00, 102.46, 90.00 |
Refinement procedure
| Resolution | 31.641 - 2.090 |
| R-factor | 0.1784 |
| Rwork | 0.176 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jfk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.943 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.600 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.070 | 0.573 |
| Rmeas | 0.090 | 0.743 |
| Number of reflections | 50379 | 3741 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.16 M Calcium acetate 0.08 M Sodium cacodylate PH 6.5 14.4% (W/V) PEG 8000 20% (V/V) Glycerol |
