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6JFM

Mitofusin2 (MFN2)_T111D

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U1
Synchrotron siteSSRF
BeamlineBL17U1
Temperature [K]100
Detector technologyPIXEL
Collection date2017-11-18
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.97914
Spacegroup nameP 1 21 1
Unit cell lengths44.437, 126.563, 79.972
Unit cell angles90.00, 102.46, 90.00
Refinement procedure
Resolution31.641 - 2.090
R-factor0.1784
Rwork0.176
R-free0.22930
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6jfk
RMSD bond length0.008
RMSD bond angle0.943
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwarePHENIX ((1.14_3260: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]126.6002.140
High resolution limit [Å]2.0902.090
Rmerge0.0700.573
Rmeas0.0900.743
Number of reflections503793741
<I/σ(I)>10.6
Completeness [%]98.8
Redundancy4.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP293.150.16 M Calcium acetate 0.08 M Sodium cacodylate PH 6.5 14.4% (W/V) PEG 8000 20% (V/V) Glycerol

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